| IgG2

Immunoglobulin G (IgG) is one of the most abundant proteins in human serum, accounting for about 10–20% of plasma protein.Immunoglobulin G-antibody responses to bacterial capsular polysaccharide antigens can be almost completely restricted to IgG2, and IgG2 deficiency may result in the virtual absence of IgG anti-carbohydrate antibodies, although these responses can also be compensated for by enhanced levels of other IgG subclasses, in particularly by elevated IgG1 and IgG3 levels. An increased susceptibility to certain bacterial infections is associated with IgG2 deficiency, suggesting a role of IgG2 in the defense to these pathogens. Low concentrations of IgG2 often occur in association with a deficiency in IgG4 and/or IgA1 and IgA2.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (μg/mL)	100	50	25	12.5	6.25	3.12	1.56	0
OD	2.11	1.26	0.8	0.5	0.33	0.24	0.19	0.08
Corrected OD	2.03	1.18	0.72	0.42	0.25	0.16	0.11	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human IgG2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	81-101	96
Plasma(n=8)	92-105	101

 

| Linearity

Samples were spiked with high concentrations of Human IgG2 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)
1 : 2	Range (%)	83-95	88-96
	Average (%)	91	93
1 : 4	Range (%)	89-104	87-108
	Average (%)	93	98